Add 5 μl SafeViewTM Classic / SafeViewTM FireRed to the gel solution.ģ. Prepare a 100 ml agarose or polyacrylamide solution.Ģ. Protocol SafeView™ Classic and SafeView™ FireRed (Protocol )ġ. Note: All Safeview™ Classic, SafeView™ FireRed, and Safeview™ Plus will work with blue light.
Unique: SafeView™ Classic is the only nucleic acid stain that can differentiate DNA and RNA in gels, by staining DNA green (max emission 520 nm) and RNA red (max emission 635 nm).SafeView™ FireRed Detect as little as 0.3 – 0.6 ng of DNA per gel band. S afeView Plus™ Detect as little as 0.05 – 0.1 ng of DNA per gel band. SafeView™ Classic Detect as little as 0.1 – 0.3 ng of DNA per gel band. SafeView™ can be viewed under UV light or blue light View and document your results as you would with EtBr staining. Easy to Use: SafeView™ directly replaces EtBr.This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis. SafeView™ are a new and safe nucleic acid stains for the visualization of nucleic acids in agarose and polyacrylamide gels.
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